Figure 9
The processes of R45F08-Gal4 -labeled neurons display a topological organization in the central complex at 50 after puparium formation (apf). Embryonically induced flip-out clones of dorsomedial (DM) lineages DM1 to DM3 and DM6 showing distinct arborization pattern by R45F08-Gal4-labeled neurons in the protocerebral bridge, the fan-shaped body and the ellipsoid body of the central complex. R45F08-Gal4 flip-out clones labeling (green), Nc82 labeling of neuropile (magenta). Maximum intensity projections of few adjacent confocal slices taken at different depths. (A-A”’) DM1-derived midline associated cells projecting most medially on the ipsilateral side of the protocerebral bridge and the fan-shaped body and additionally innervating on the contralateral side of the fan-shaped body (most laterally) and the ellipsoid body. (B-B”’) DM2-derived midline associated cells projecting adjacent to DM1 more laterally on the ipsilateral side of the protocerebral bridge and the fan-shaped body and additionally innervating on the contralateral side of the fan-shaped body (medially adjacent to DM1) and the ellipsoid body. (C-C”’) DM3-derived midline associated cells projecting adjacent to DM2 more laterally on the ipsilateral side of the protocerebral bridge and the fan-shaped body and additionally innervating on the contralateral side of the fan-shaped body (medially adjacent to DM2). DM3 innervates the ellipsoid body on the ipsilateral side. (D-D”’) DM6-derived midline associated cells projecting most laterally on the ipsilateral side of the protocerebral bridge and the fan-shaped body and additionally innervating on the contralateral side of the fan-shaped body (most medially). DM6 innervates the ellipsoid body on the ipsilateral side. EB, ellipsoid body; FB, fan-shaped body; lrFB, lateral root of fan-shaped body; NO, noduli; PB, protocerebral bridge; p, peduncle. Neuroanatomical nomenclature according to[3, 40]. Scale bars 25 μm.