Figure 7

Differentiation and integration of larval fan-shaped body primordium-innervating neurons into the mature central complex. Spatiotemporal analysis of the R45F08-Gal4 expressing cells and neurites as well as their innervation of developing central complex substructures at 30 h and 50 h of metamorphosis and in the adult. R45F08-Gal4 labeling (green), (A-B”’) DN-cadherin labeling of neuropile (magenta), (C-C”’) Nc82 labeling of neuropile (magenta). Maximum intensity projections of few adjacent confocal slices taken at different depths. (A-A”’) At 30 h after puparium formation (apf) the R45F08-Gal4-labeled cells as well as their innervation of the developing fan-shaped body are similar to that observed at 24 h apf. (B-B”’) At 50 h apf, the innervation of the developing fan-shaped body by R45F08-Gal4-labeled cells continues to manifest eight columnar domains and labeled processes are also seen in the inner and outer layers of the ellipsoid body and in the bulbs. (C-C”’) In the adult the innervation of the mature fan-shaped body by R45F08-Gal4-labeled cells is largely restricted to two well separated layers, each of which is subdivided into the eight major columnar domains. Labeled processes remain visible in the ellipsoid body layers and in the bulbs. The noduli are not innervated by R45F08-Gal4-labeled cells at any stage. In (A”-C”) asterisks indicate one labeled columnar domain in two layers of the fan-shaped body. BU, bulbs, CX, calyx; dlrFB, dorsolateral root of fan-shaped body; EB, ellipsoid body; FB, fan-shaped body; LAL, lateral accessory lobe; lrFB, lateral root of fan-shaped body; mrFB, medial root of fan-shaped body; NO, noduli; PB, protocerebral bridge; p, peduncle. Neuroanatomical nomenclature according to[3, 40]. Scale bars, 25 μm.