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Figure 2 | Neural Development

Figure 2

From: Early-born neurons in type II neuroblast lineages establish a larval primordium and integrate into adult circuitry during central complex development in Drosophila

Figure 2

Clusters of midline associated neurons and the fan-shaped body primordium are revealed by Gal414-94driven labeling of dorsomedial (DM) lineages in the late larval brain. (A) Distal cell clusters corresponding to DM neuroblasts and their recently born progeny as well as a cluster of midline associated cells in close proximity to the fan-shaped body primordium are labeled by Gal414-94. The dotted line indicates midline associated cells and primordium. DM1 to DM6 are indicated with numbers; lat, lateral type II lineages. Maximum projection of all stacks. (B,B’) The Gal414-94-labeled fan-shaped body primordium (green) is colabeled with the neuropile marker DN-cadherin (magenta/white). (C-C”’) The Gal414-94-labeled fan-shaped body primordium (green) is not colabeled with the synaptic marker Bruchpilot (magenta/white). (D,D’) The Gal414-94-labeled fan-shaped body primordium (green) is colabeled with the membrane marker Neurotactin (magenta/white). (E-J) Single sections at different depths show the GAL414-94-labeled cells of type II lineages (green) in a brain hemisphere colabeled for Neurotactin (magenta) revealing the relative position of the midline associated cells, the neurite fascicles of the DM lineages, and the fan-shaped body primordium (FBpr). DM1 to DM6 are indicated with numbers; lat, lateral type II lineages. Circles in (I) indicate neurite fascicles. (K-K”) GAL414-94-labeled cells and projections of type II lineages (green) at the midline colabeled for DN-cadherin (magenta). The two bilateral clusters of midline-associated cells (K) give rise to four neurite fascicles per hemisphere (K’), which project into the fan-shaped body primordium (K”). The fan-shaped body primordium is composed of four subcompartments per hemisphere (arrowheads) (K”). FBpr, fan-shaped body primordium; dlrFB, dorsolateral root of fan-shaped body; mrFB, medial root of fan-shaped body; lrFB, lateral root of fan-shaped body; nomenclature according to[40]. (B-D’, K-K”) are maximum intensity projections of few adjacent confocal slices. (A) and (E-K”) scale bars, 25 μm, (B-D’) scale bars, 10 μm.

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