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Figure 6 | Neural Development

Figure 6

From: Histamine up-regulates fibroblast growth factor receptor 1 and increases FOXP2 neurons in cultured neural precursors by histamine type 1 receptor activation: conceivable role of histamine in neurogenesis during cortical development in vivo

Figure 6

Chlorpheniramine prevents FOXP2 neuronal differentiation i n vitro and in vivo . (A) Significant increase of mature MAP2+ neurons positive for FOXP2 after HA stimulation of cultured NPC. This induction was blocked by co-incubation of HA with chlorpheniramine (Chlorph.). *P <0.01 versus all the remaining conditions. (B to E) Representative micrographs for control (-HA, B), 100 μM HA (C), 100 μM HA + 1 μM chlorpheniramine (D) and chlorpheniramine alone (E). (F to H) Effect of injection of chlorpheniramine in E12 developing rat brains that were analyzed two days later. (F) Scheme of a coronal section from an E14 rat telencephalon. The arrow represents the area used for analysis. The red line represents the section of the dorsal telencephalon used for in vitro experiments. (G and H) Representative images of coronal sections stained with antibodies for β-tubulin III and FOXP2. In control conditions (G), numerous cells are positive for these markers. In contrast, after injection of 25 μg of chlorpheniramine (H), a decrease in both β-tubulin III and FOXP2 was found. This effect was observed in four control and four chlorpheniramine-injected embryos from different dams. Scale bar = 100 μm. E, embryonic day; HA, histamine; MAP2, microtubule associated protein 2; NPC, neural precursor cells.

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Neural Development

ISSN: 1749-8104