Figure 2
Functional and morphological properties of green fluorescent protein (GFP) expressing interneurons in dorsal lateral geniculate nucleus (dLGN). (A,B) Plots showing the resting membrane potential (RMP; (A)) and input resistance (Ri; (B)) for a total of 72 interneurons recorded at different postnatal weeks (week 1, n = 6 cells, 1 slice; week 2, n = 15 cells, 10 slices; week 3, n = 27 cells, 14 slices; week 4, n = 14 cells, 4 slices; week 5, n = 10 cells, 2 slices). (C) Examples of voltage responses to varying current injection (indicated on the far right) at P10. The letters next to the traces correspond to some of the more salient active membrane properties of interneurons (h, hyperpolarization activated mixed cation conductance; LT, rebound low threshold Ca2+ spike; A, slow outward rectifying K+ conductance). The inset shows the linear relationship between spike frequency and current injection for responses in (C). (D) Example of excitatory postsynaptic responses recorded at −65 mV in current (top, excitatory postsynaptic potentials (EPSPs)) or voltage clamp (bottom, excitatory postsynaptic currents (EPSCs)) of a P14 interneuron evoked by increasing levels of electrical stimulation (40, 45, 50, 55, and 60 μA).