Figure 1

Distribution of green fluorescent protein (GFP) expressing interneurons in dorsal lateral geniculate nucleus (dLGN). (A) Coronal section of dLGN from a P7 GAD67-GFP mouse showing the distribution of GFP-expressing interneurons in thalamus (vLGN, ventral lateral geniculate nucleus; VB, ventrobasal complex). (B) Coronal section of dLGN from a P26 GAD67-GFP mouse that had one eye injected with cholera toxin subunit B (CTB) conjugated to Alexa Fluor 594. Interneurons in dLGN express GFP (green) and uncrossed retinogeniculate projections from the ipsilateral eye were anterogradely labeled with CTB (red). (C) Thalamic slice preparation at P11 showing z-stack projection image of GFP-expressing interneurons in dLGN labeled with biocytin during intracellular recordings. (D) Parasagittal section of P14 dLGN with biocytin labeled interneurons. Dashed lines outline the borders of dLGN and the region that corresponds to the region occupied by ipsilateral projections. (E) Z-stack projection images of GFP-expressing interneurons at P8 and P17 that were filled with biocytin during intracellular recording and then reconstructed using confocal microscopy (see Krahe et al. [10] for details). Scale bars, 100 μm. In all, 22 interneurons were labeled with biocytin (P7, n = 6 cells, 3 slices; P8, n = 1 cell, 1 slice; P10, n = 4 cells, 2 slices; P11, n = 2 cells, 1 slice; P14, n = 3 cells, 1 slice; P17, n = 3 cells, 3 slices; P20, n = 2 cells, 2 slices; P27, n = 1 cell, 1 slice).