Figure 1

Mosaic labelling techniques in the zebrafish embryo. (A, C) Visualisation of individual transplanted retinal cells from donor embryos labelled with either H2B-RFP and membrane tagged-GFP (A) or the Atoh7-gap43RFP transgene (C) at 28 hpf. In (C) frames from a time-lapse series showing apical process retraction from the apical surface (up, see vertical dotted line) and axon extension at the basal surface (white arrow) of the s2 sibling that differentiates into a retinal ganglion cell after asymmetric division. R, Retina; L, lens. Scale bar is 10 μm. (B) Few small cell clones labelled by electroporation of CMV:mCitrine. Acquired images were pseudo-coloured afterwards according to z-level to visualize individual cell clones in the brain at 32 hpf. In (A, C), the retina is oriented dorsal up. (B) Dorsal view of the midbrain. The data presented in this figure were acquired by the course participants with the help of Wiebke Sassen (Braunschweig), Shahad Albadri and Anne-Laure Duchemin (Heidelberg).