Figure 3

GFP-TOG primarily localizes to microtubule (MT) lattice yet rescues MT plus-end velocity defect of XMAP215. (A) Micrograph of XMAP215-GFP in neuron, with arrowhead pointing to plus-end tracking and arrows to lattice-binding. (See Additional file 3A) (B) Micrograph of GFP-TOG in neuron, with arrow pointing to lattice-binding. (See Additional file 3B) (C) Timelapse montage of representative MT, with mKate2-EB1 (top) and GFP-TOG (bottom). (See Additional file 3C) (D) Quantification of GFP-TOG localization in growth cones and neural crest. n = number MTs examined. (E-G) Quantification of MT parameters. Box-and-whisker plots indicate the mean (diamond), median, extrema and quartiles. *P < 0.05 comparing conditions. ns not significant. n = axonal growth cone number. Bar is 10 μm for (A-B), 1 μm for (C).