Figure 1

EphB2 expression at P7. EphB2 is expressed in the VCN (asterisk, A) and in the MNTB (B) at P7. Immunohistochemistry results (A-B) were confirmed with X-gal histochemistry on tissue from P7 EphB2^lacZ/lacZ mice (C-F). X-gal staining is present in VCN (asterisk, C) and in axons and calyces in MNTB (D and E). No axonal staining is present at the midline (arrow, E), where VCN axons cross to reach the contralateral MNTB. VCN axons in the ventral acoustic stria (arrowheads, F and G), a population of proximal uncrossed VCN axons, also lack staining (black arrowheads, F). Unilateral VCN NeuroVue dye labeling is shown in panel (G) to demonstrate the location of the ventral acoustic stria (white arrowheads) in panel (F) in relation to VCN (asterisks, F and G) and lateral superior olive (LSO). Scale bars in panels B (also applies to D) and E represent 100 μm3