Figure 8

Mixed neuronal/neuroendocrine mRNA expression pattern in P21 adrenal medulla after conditional Dicer inactivation. (A) Adrenal medulla in a mouse with conditional Dicer 1 ablation is shown by DBH ISH (white arrowhead). (B) NF-L and (C) NF-M mRNA expression is detected in a substantial fraction of cells in the remaining adrenal medulla, and (D) SCG10 signals are well above background. Prominent signals are observed for (E) Snap25, (F) Syt1 and (G) Syt7 mRNAs, and (H) Rab3a-positive cells are occasionally detected. Adjacent sections were used for ISH with the probes indicated, and experiments were performed to compare three mutant and three control animals for each individual probe. The panels from a representative homozygous mutant animal shown in this figure can be directly compared with those in Figure 2 from a representative control animal. Scale bar: 100 μm. DBH, dopamine β-hydroxylase; ISH, in situ hybridization; NF-L, low molecular weight neurofilament protein; NF-M, medium molecular weight neurofilament protein; P21, postnatal day 21; SCG10, superior cervical ganglia, neural specific 10; Snap25, synaptosomal-associated protein 25; Syt1, synaptotagmin I; Syt7, synaptotagmin VII.