Figure 9

GABAergic spinal commissural axon trajectory is normal in the E10.5 Npn2^−/−spinal cord. (A-C), A wild type E10.5 transverse spinal cord section at the cervical level is double-labeled with anti-GAD65 (A, green) and anti-TAG1 (B, red), or merged of the same section (C). At this developmental stage, GABAergic commissural neurons in the ventromedial spinal cord can be prominently labeled and a few axons have projected across the floor plate to the contralateral side. (D-F), An Npn2^−/− E10.5 cervical spinal cord section from the same litter is double-labeled with anti-GAD65 (D, green) and anti-TAG1 (E, red), or merged of the same section (F). Both GAD65- and TAG1-positive commissural axons are seen to project across the ventral midline in the Npn2^−/− spinal cord. Scale bar in F, 25 μm for A-F.