Figure 7

Malposition of IGL neurons in vldlr^−/−; lrp8^−/−mutant LGN. (A,B) NPY-expressing neurons, which are normally restricted to the IGL, were immunolabeled in (A,D) P14 control and (B,C,E,F) vldlr^−/−;lrp8^−/− mutant (DKO) LGN. In (A to C), LGN neurons were colabeled by NeuN-IHC. In (D to F), retinal arbors were colabeled by binocular injection of Alexa Fluor 594 CTB. Insets show high magnification images of the boxed areas in (A to F). Note the presence of NPY-immunoreactivity in the dorsomedial pole of vldlr^−/−;lrp8^−/− mutant dLGN (as seen in insets). NPY-immunoreactivity also extended into the vLGN in vldlr^−/−;lrp8^−/− mutant LGN (arrowheads). For all images, the outline of the dLGN is depicted with white dots. dLGN are labeled ‘d’, vLGN are labeled ‘v’ and arrows indicate IGL. Scale bar = 180 μm for (A to F) and 50 μm for insets. CTB, cholera toxin B subunit; DKO, double knockout; dLGN, dorsal LGN; IGL, intergeniculate leaflet; IHC, immunohistochemistry; LGN, lateral geniculate nucleus; Neu-N; neuronal nuclei; NPY, neuropeptide Y; vLGN, ventral LGN.