Figure 4

Development of retinogeniculate projections in wild-type, vldlr^−/−, lrp8^−/−and vldlr^−/−; lrp8^−/−mice. Retinogeniculate projections in (A) P3 controls, (B) P3 lrp8^−/− mutants, (C) P4 vldlr^−/− mutants and (D) P3 vldlr^−/−;lrp8^−/− mutants were labeled by intraocular injection of fluorescently conjugated CTB. In (D), note the ectopic region of binocular retinal input in the dorsomedial pole of vldlr^−/−;lrp8^−/− dLGN is labeled with arrowheads. (E,F) High magnification images of the areas indicated by boxes in (A) and (D), respectively. Left eyes were injected with Alexa Fluor 594 CTB and right eyes were injected with Alexa Fluor 488 CTB. LGN from right hemispheres are shown. ‘Contra’ denotes projections originating from the contralateral retina and ‘ipsi’ denotes projections originating from the ipsilateral retina. dLGN are labeled ‘d’, vLGN are labeled ‘v’ and arrows indicate IGL. The color of the label in panels (A to D) and (A’ to D’) indicates the color of the image shown in (A” to D”). Scale bar = 200 μm for (A to D) and 75 μm for (E,F). CTB, cholera toxin B subunit; dLGN, dorsal LGN; IGL, intergeniculate leaflet; LGN, lateral geniculate nucleus; vLGN, ventral LGN.