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Figure 6 | Neural Development

Figure 6

From: The Caenorhabditis elegans voltage-gated calcium channel subunits UNC-2 and UNC-36 and the calcium-dependent kinase UNC-43/CaMKII regulate neuromuscular junction morphology

Figure 6

unc-43 can cell autonomously regulate presynaptic morphology. (A) The unc-43(e408) loss-of-function (LOF) mutation caused an increase in puncta size. (B) The e408 mutation suppressed the morphological defects found in the nid-1 background. (C) The n498 gain-of-function (GOF) mutation in unc-43 caused enlarged puncta that were disorganized. (D-F) Expression of an mCherry-tagged UNC-43 chimera specifically in the GABAergic neurons largely rescued the SNB-1::GFP morphology defects present in e408 animals. (G) A line scan of the VD12 axon region (line in panel D) demonstrating the co-incidence of mCherry::UNC-43 (red) with SNB-1::GFP (green). GFP/RFP coincidence was measured in a single confocal slice. (H) A plot of the puncta area measured by genotype as mean (circle) with the 5 to 95% confidence interval (whiskers). All animals in these micrographs are young adults (1 to 2 days post 4th larval stage (L4)).

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Neural Development

ISSN: 1749-8104