Figure 2

Wild type (wt) animals have enlarged puncta during late 4th larval stage (L4) development. During the early (A) and mid (B) L4 stage SNB-1::GFP puncta in wild type animals were evenly sized and spaced. (C) But during late L4 puncta were often enlarged and/or elongated (arrow) along the axon. (A’-C’) Representative examples of vulval progression used to mark transition from early (A’), mid (B’) and late (C’) phases of L4 development. (D) In young adults puncta were again evenly sized and spaced. In unc-2 mutants the early (E) and mid (F) L4 stage animals had evenly sized and spaced puncta, that appeared enlarged in late L4 (G), but not elongated. (H) Puncta remained enlarged in young e55 adults, compared to those in equivalently aged wild type animals (D). (I) Plots of the puncta length in the different stages are presented as means (circles) with the 5 to 95% confidence intervals (whiskers). During late L4 of wild type (area also shaded for comparison), puncta are longer, and more variable in length, than during other periods imaged. By comparison, during late L4, although longer than wild type adults, unc-2 mutants had only a modest increase in length. (J) A plot of the length of each puncta divided by the width for each genotype.