Figure 6

MBL-1 is required cell autonomously in DA9 and is expressed in many dorsal cord neurons. (A) Genomic region of the X chromosome including mbl-1. mbl-1(tm1563) deletion is indicated above a schematic of genomic region. All cDNA isoforms were reported by Sasagawa et al. [31]. Bottom: schematic of genomic rescuing constructs. Black boxes represent exons and thin black lines represent introns. Thin gray lines indicate splicing patterns. (B) Quantification of the number of DA9 synapses present in the dorsal cord. Two independent transgenic lines were scored for each rescuing construct. Error bars represent standard error of the mean. ***P < 0.0001, t-test. (C-H) Endogenous expression of MBL-1 (magenta) and co-localization with A-type motorneurons (green). Transgene used is wyEx4015. MBL-1 is expressed in the posterior ventral cord neurons DA8, DA9, VA12, and VA11 (C-E). (F-H) MBL-1 is expressed in A-type motorneurons. DAs can be identified by their commissure crossing the midline of the animals. Scale bar represents 10 μm.