mbl-1 disrupts synaptic vesicle localization in the motorneuron DA9. (A-C) Localization of synaptic vesicle-associated GFP::RAB-3 in adult wild type (wyIs85) (A), mbl-1(wy560) (B) and mbl-1(tm1563) (C) mutants. Animals are oriented such that anterior is to the left and dorsal is up. Synaptic region (square bracket), dendrite (curly bracket) and DA9 cell body (asterisk) are indicated. Scale bar represents 10 μm. (D) Quantification of the number of axonal RAB-3 puncta observed by epifluorescence microscopy. Error bars represent standard error of the mean. ***P < 0.0001, t-test. (E-G) Confocal micrographs of ten wild-type (wyIs85), mbl-1(wy560) or mbl-1(tm1563) animals, straightened and aligned along their anteroposterior axes. Scale bar equals 10 μm. Anterior is to the left. (H) Quantification of fluorescence intensity along the x-axis of the composite images. Dotted lines represent actual values and solid lines represent a 10-μm sliding average of fluorescence intensity. (I) Percentage of animals with visible puncta in the dendrite in wild-type, mbl-1(wy560) and mbl-1(tm1563) animals.