Figure 8
From: Bilateral enucleation alters gene expression and intraneocortical connections in the mouse
Analysis of somatosensory INC position within the medial parietal cortex of control and enucleated mice. Following placement of DiA or DiI crystals into the somatosensory cortex and time for retrograde transport of tracer, the straight-line distance from the midline edge of layer 1 of the neocortex to the most medial retrogradely labeled cell was measured using precision micrometry. Enucleated animals exhibited a distinct extension of labeling from the somatosensory cortex into the visual cortex, resulting in a reduced distance to midline (0.46 ± 0.04 mm; df = 12; n = 7) when compared to controls (0.97 ± 0.04 mm; df = 12; n = 7; P < 0.01; two-sample t-test). DiA: 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodide; DiI: 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; INC: ipsilateral intraneocortical connection.