Figure 5
From: Bilateral enucleation alters gene expression and intraneocortical connections in the mouse
P0 enucleation does not dramatically alter the sensory thalamic afferent position at P10. High magnification views of 100 μm coronal hemisections of DAPI stained DiI/DIA labeled sections and Nissl sections through the thalamus of (A1-B1) P10 control and (A2-B2) enucleated brains. Retrograde thalamocortical labeling in the dLGN and VPN was observed for both (A1) control and (A2) experimental brains, which confirmed that DPLs were within the visual and somatosensory cortices and demonstrated no effect of enucleation. Alternate sections from P10 (B1) control and (B2) enucleated brains stained for Nissl substance to aid in the verification of the nuclear retrograde label. The dLGN and VPN are indicated with text and outlined in the Nissl sections. Dorsal (D) is up and medial (M) is to the left. Scale bar = 500 μm. DAPI: 4',6-diamidine-2-phenylindole dihydrochloride; dLGN: dorsal lateral geniculate nucleus; DiA: 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodide; DiI: 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; DPL: dye placement location; P: postnatal day; VPN: ventral posterior nucleus.