Figure 2
From: Bilateral enucleation alters gene expression and intraneocortical connections in the mouse
Neocortical expression of six genes at P10 in the control and enucleated mice. In situ RNA hybridization was used to determine the distribution of transcripts for (A1-A8) ephrin A5, (B1-B8) Id2, (C1-C8) Cad8, (D1-D8) COUP-TF1, (E1-E8) Lhx2 and (F1-F8) RZRß at P10 in control (left panel, columns 1-4) and enucleated (right panel, columns 5-8) brains as labeled. All panels are low magnification views of one hemisphere after sectioning at 100 μm in the coronal plane, shown in a rostral to caudal (left to right) series for each gene. Ephrin A5 expression in brains from control animals (A1-A4) was found predominantly in the parietal and occipital cortex, with noted exclusion medially (arrow in A2). In brains from experimental animals, an atypical medial expansion of ephrin A5 expression was observed in the parietal cortex (arrow in A6). Control Id2 expression was observed throughout the rostral/caudal extent of the cortex with very low levels in layer 4 (B1-B4), with no major changes observed in enucleated brains (B5-B8). Cad8 expression was seen in a complex pattern with robust expression in several domains medially and laterally (C1-C4), with no change in expression in enucleated brains (C5-C8). COUP-TF1 expression was observed in a gradient throughout the cortex (D1-D4) and the level and distribution of transcripts observed in experimental brains (D5-D8) did not differ significantly from controls. Lhx2 is present at low levels in superficial cortical layers in both control (E1-E4) and experimental (E5-E8) brains. RZRß showed robust expression in layers 4/5 at all levels of the cortex (F1-F4), and this expression remained unchanged after enucleation (F5-F8). All sections are oriented with dorsal (D) up and lateral (L) to the right. Scale bar = 1,000 μm. Cad8: Cadherin-8; COUP-TFI: chicken ovalbumin upstream promoter transcription factor 1; dLGN: dorsal lateral geniculate nucleus; Id2: inhibitor of DNA binding 2; Lhx2: LIM homeobox protein 2; P: postnatal day; RZRß: retinoic acid receptor related orphan receptor beta.