Figure 6

Hypothalamic neurons derived from progenitors that express Shh after E10.5 settle in medial and lateral aspects of the tuberal hypothalamus. (A-F) Early and late stage fate-mapped cells contribute to the lateral tuberal and mammillary hypothalamus. Ventro- and dorsolateral areas of the hypothalamus are circled with a dashed line. (G-I) Immunostaining for fate-mapped cells (β-gal, red) and NeuN, a pan-neuronal marker (green). Pictures were taken using a Zeiss Apotome set-up. Late-stage fate-mapped cells overlap with the neuronal marker NeuN and have neuronal morphology (H). Red arrowheads indicate neuronal processes. Scale bar: 800 μm (A-F); 100 μm (G, I, J); 20 μm (H).