Midbrain GABAergic neurons are not affected in Uncx4.1- deficient embryos. (A-B) In situ hybridization (ISH) of Helt on E11.5 coronal sections reveals no difference in the absence of Uncx4.1. (C-F) The GABAergic marker GAD67 is expressed normally in Uncx4.1−/−embryos at E11.5 (C-D) and E17.5 (E-F) compared to control animals. (G-J) Immunohistochemistry (IHC) against Olig2 shows no difference in control and Uncx4.1−/−embryos at E11.5 (G-H) and E16.5 (I-J). E, embryonic day.