Developmental profile of Shank1 at IHCs and OHCs in the organ of Corti. A-D Example fluorescent whole-mount images revealing immunolabelling of Shank1 (green) and CtBP2/RIBEYE (red). Shank1 is evident at the base of IHCs from P0 through to adulthood. Shank1 is significantly weaker in the OHCs. The strongest Shank1 expression is seen in OHCs at P3 followed by downregulation such that in the adult no Shank1 was observed. Scale bar 10 mm. Grey dotted lines delineate the OHC and IHC regions. In each image, the area denoted by the white square is shown in the magnified form below: Shank1 alone (green, LHS) and overlay of Shank1 and CtBP2 (red, RHS). E-J Quantification of total Shank1 puncta number (E,H), Shank1 puncta volume (F,I) and per cent Shank1 co-localised with CtBP2/RIBEYE (G,J) in IHCs and OHCs from P0 to adult (n = 6 for all age groups). *p < 0.05, **p < 0.01, **p < 0.005.