Figure 5
From: Synaptic profiles during neurite extension, refinement and retraction in the developing cochlea
Changes in GluA2/3 subunits in IHCs and OHCs during development. A-E Example fluorescent whole-mount images of GluA2/3 (green) and CtBP2/RIBEYE labelling (red). Scale bar 5 mm. Grey dotted lines delineate the OHC and IHC regions. Boxed regions from both the IHC (left) and OHC (right) regions are shown below each image for each age. F Quantification of the total number of GluA2/3 puncta in IHCs between P0 and adult. G Quantification of the developmental changes in synaptic GluA2/3 in IHCs, as assessed by per cent co-localisation with CtBP2/RIBEYE puncta. H Quantification of the total number of GluA2/3 puncta in OHCs between P0 and adult. I Quantification of the developmental changes in synaptic GluA2/3 in OHCs, as assessed by per cent co-localisation with CtBP2/RIBEYE puncta. In all graphs, data are represented as mean ± SEM; n ≥ 5 in all age groups. *p < 0.05, ***p < 0.001.