Figure 4
From: Synaptic profiles during neurite extension, refinement and retraction in the developing cochlea
Developmental changes in presynaptic Bassoon in IHCs and OHCs. A-E Maximal projection confocal images of the Organ of Corti in cross section illustrating the immunofluorescent localisation of Bassoon (green) and CtBP2/RIBEYE positive puncta (red) superimposed on transmitted light images for anatomical reference from P0 to adult. Scale bar 10 mm. F-J Fluorescence images of Bassoon and CtBP2/RIBEYE immunostaining, without the transmitted light images, to highlight the developmental changes. K-P Quantification of the developmental changes in the total number of Bassoon puncta (K,N), the percentage of synaptic Bassoon puncta as measured by co-localisation with CtBP2 puncta (L,O), and the intensity changes in synaptic Bassoon puncta (M,P), in IHCs (K,L,M) and OHCs (N,O,P) respectively. In all cases n = 6 animals. *p < 0.05, **p < 0.005, ***p < 0.001.