Figure 2

RabGDI is required for the detection of negative cues at the midline. In the absence of Axonin-1/NrCAM interactions, some axons failed to enter the floor plate (indicated by dashed lines) and turned prematurely into the longitudinal axis along the ipsilateral floor-plate border (arrow in A). This Axonin-1/NrCAM phenotype was partially rescued when RabGDI was downregulated together with Axonin-1 and NrCAM (B,C). Similarly, the concomitant silencing of Axonin-1/NrCAM resulted in a partial rescue of the axon stalling phenotype observed following downregulation of RabGDI alone (C). Quantification of the phenotypes (see Methods) is shown in (C). n.s. = not significant, * p<0.05, ** p<0.01, *** p<0.001. Graphs show mean + SEM in each case. Bar: 50 μm.