Figure 4

Mitochondria and Apc2-GFP have characteristic localizations in dendrites throughout development. (A) Mitochondria were labeled with mitoGFP and cell shape was outlined with mCD8-RFP. Images of mitochondrial localization in living embryos and larvae were acquired at different stages of development. (B) Apc2-GFP was expressed in neurons together with mCD8-RFP. Images of dendrites at different stages were acquired in whole, living embryos or larvae. (C) Images of mitochondria or Apc2-GFP were used to quantitate the percent of ddaE branch points occupied with green fluorescence. Only branch points along the main trunk of the comb dendrite of ddaE were counted. % occupancy was calculated for each cell, and averages are shown. The error bars show standard deviations. Mitochondria lengths were also measured at different stages of neuronal development (middle graph); averages are shown with standard deviations indicated by the error bars. Numbers on the bars indicate numbers of animals analyzed at each time point. Statistical significance was calculated with a t-test.