Figure 3

Ribosomal protein L10 localizes to dendrite branch points throughout dendrite development. The ribosomal protein L10 tagged with YFP was expressed in neurons together with mCD8-RFP to mark cell shape. Patterns of fluorescence were analyzed at different stages of dendrite development. Examples from different stages are shown in (A). Arrows point out the cell body of the ddaE neuron, and arrowheads point to spots of YFP at dendrite branch points. (B) The percent of proximal branch points occupied by ribosomes was counted. The percent of branch points occupied was calculated for each cell. The average of these numbers is shown in the graph. The error bars show the standard deviation. The data on occupancy of distal branch points were pooled from all cells as numbers were smaller. Numbers on the bars indicate the number of animals analyzed at each time point. (C) An example of a FRAP experiment is shown. The same ddaE cell is shown in the top and bottom rows. An arrow points to the area that was bleached. In the pre-bleach image a bright spot of L10-YFP is present at the branch point that was bleached. Fluorescence of both RFP and YFP is strongly reduced after bleaching. However, by 6 s the mCD8-RFP has started to return to the bleached area. Very little L10-YFP returned to the bleach area during the time course. (D) The average fluorescence recovery of nine cells is shown in the graph. Error bars show standard deviations.