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Table 1 Gain of Mdka function does not advance cell cycle exit

From: Midkine-A functions upstream of Id2a to regulate cell cycle kinetics in the developing vertebrate retina

 

Average number of cells/section at 34 hpf

EdU-negative cells at 48 hpf (n)

Proportion of EdU-negative cells at 48 hpf

Untreated

156 ± 22

53 ± 10

0.34 ± 0.09

Mdka l-o-f

138 ± 19

2 ± 2

0.02 ± 0.02a

WT

147 ± 23

51 ± 38

0.34 ± 0.17

Mdka g-o-f

191 ± 32

71 ± 28

0.38 ± 0.08

  1. For each group, half the embryos were sacrificed at 34 hpf for cell counts. The remaining embryos were injected with EdU every 6 h starting at 34 hpf and fixed at 48 hpf. EdU-negative cells counted at 48 hpf were those that had exited cell cycle by 34 hpf. The proportion of EdU-negative cells was not significantly different between the untreated, WT, and Mdka g-o-f groups. The proportion of post-mitotic cells in the Mdka l-o-f group was significantly smaller than in controls. g-o-f, gain-of-function; l-o-f, loss-of-function. aP <0.05.