Figure 8

Dorsal view of neuroblast clones showing Ultrabithorax expression and genetic manipulation in lineage 12. Insets show either Ubx immunostaining (magenta) of sections at mid-clone (A’-H’) or adjacent to the neuroblast (A”-H”); or neurotactin immunostaining (red): single confocal slices shortly after the axon bundle enters the neurotactin scaffold (M’-P’). (A-D) Wild-type clones. (C) Only bundle 12c is present in T3. (D) Only 12id is present in A1. (E-H) dronc^- clones. (G) Bundles 12c and 12id, but not 12im, are present in T3. (H) Bundles 12c and 12id, but not 12im, are present in A1. (I-L) Ubx^- clones. (K) Bundles 12im, id, and c are present in T3. (M-P) UAS-Ubx clones. Cell bodies are missing and axon bundles reduced or missing in all segments. (M’-P’) Lineage 12 is still identifiable based on characteristic position in neurotactin scaffold. White or green, anti-CD8, Ubx: Ultrabithorax.