Figure 3

Clonal analysis of apoptosis-blocked dorsomedial (DM) lineages during larval development. (A) Schematic representation of the six DM lineages located at the posterior dorsomedial edge of the brain hemisphere. Cell number was quantified for the DM1 lineage (marked in green). (B) Average number of cells in wild-type (light blue) and apoptosis-blocked (dark blue) DM1 lineage. (C) Average number of glial cells in wild-type (light blue) and apoptosis-blocked (dark blue) DM1 lineage. (D-F) A wild-type mosaic analysis with a repressible cell marker (MARCM) clone of a DM1 lineage; cells labeled with mCD8::GFP (green). Single confocal sections at the level of (D) the neuroblast (arrowhead), and (E) the secondary axon tract (arrow), and superimposition of all single optical sections containing (F) labeled cells are shown. (G-I) A MARCM clone of a cell death-blocked DM1 lineage; cells labeled with mCD8::GFP (green). Single confocal sections at the level of (G) the neuroblast (arrowhead), and (H) the secondary axon tract (arrow), with (I) superimposition of all single optical sections containing labeled cells are shown. (B, C) Error bars represent standard deviation. Scale bars = 20 μm. Genotypes: (D-F) hs-flp; tub-Gal4 UAS-mCD8::GFP; FRT2A tub-Gal80/FRT2A; (G-I) hs-flp; tub-Gal4 UAS-mCD8::GFP; FRT2A tub-Gal80/FRT2A H99.