Programmed cell death in dorsomedial (DM) lineages occurs during both larval and pupal development. (A-D) DM lineage cells expressing activated Caspase-3 (aCasp-3) at different times during post-embryonic development. Single confocal sections show activated Caspase-3 (red) and DM lineage cells labeled with mCD8::GFP (green) at (A) 48 and (B) 96 hours after larval hatching (ALH), and (C) 12 and (D) 24 hours after puparium formation (APF). Asterisks indicate cells undergoing apoptosis. (E) Average number of observed apoptotic cells per DM lineage at different stages during larval and pupal development (error bars represent the standard error). For each time point, more than 36 DM lineages were examined. Scale bars = 10 μm. Genotype: (A-D) UAS-mCD8::GFP; erm-Gal4R09D11.