Figure 1
An increased number of neural cell somata is present in apoptosis-blocked dorsomedial (DM) lineages. (A, B) Neuropile labeled by nc82 and DM lineage-derived cells labeled with green fluorescent protein (GFP) in (A) wild-type flies and (B) DM lineage-specific apoptosis-blocked flies. Single confocal sections at the level of the fan-shaped body of the central complex are shown. (C, D) Neuropile labeled with nc82 and DM lineage-derived cell bodies labeled with GFP in (C) wild-type and (D) DM lineage-specific apoptosis-blocked flies. Single confocal sections at the level of the posterior dorsal midline are shown. Note the marked increase in the number of labeled DM lineage-derived cell somata in the apoptosis-blocked flies compared with wild-type. (A) and (C) are from the same preparation, as are (B) and (D), and both preparations are from pupae 48 hours after puparium formation. Scale bars = 20 μm. Genotypes: (A, C) UAS-flp; erm-Gal4R09D11 act>CD2>Gal4 UAS-mCD8::GFP (B, D) UAS-flp; UAS-p35; erm-Gal4R09D11 act>CD2>Gal4 UAS-mCD8::GFP.