Figure 3
From: Coordinated Eph-ephrin signaling guides migration and axon targeting in the avian auditory system
Targeting errors are increased when EphB2 is misexpressed during development. (A-C, A’-C’) RDA trace of left-sided NL in E10 embryos following E2 transfection of EGFP control (A, A’), full-length EphB2 (B, B’), or kiEphB2 (C, C’) plasmid. (A, B, C) are original 20X images with the outlined box expanded and color-inverted in (A’, B’, C’) to highlight fine processes of single axons crossing the NL cell body layer inappropriately (white arrows). (D) Quantification of the mean number of axon targeting errors per group with SEM bars. (E) Percentage of embryos in each group demonstrating atypical features of NL morphology, including a reduced length and/or a disorganized cellular layer. Scale bar = 100 μm in (A-C) and 35 μm in (A’-C’).