Figure 6

Ectopic differentiation of olfactory bulb interneurons in Neurog2^KI/KIand Neurog1/2^−/− embryos. (A) to (D) Expression of Ascl1 in embryonic day (E) 13.5 wild-type (A), Neurog1^−/− (B), Neurog2^KI/KI (C), and Neurog1/2^−/− (D) OBs. (E) to (T) Expression of Dlx1 (E) to (H), Gad1 (I) to (L), calretinin (CR) (M) to (P) and TH (Q) to (T) in E18.5 wild-type, (E,I,M,Q), Neurog1^−/− (F,J,N,R), Neurog2^KI/KI (G,K,O,S), and Neurog1/2^−/− (H,L,P,T) OBs. Blue is DAPI nuclear stain in (M) to (T). Arrowheads in (C,D) mark the upregulated expression of Ascl1 in the OB of Neurog2^KI/KI and OBLS of Neurog1/2^−/− embryos. Arrowheads in (H,L,P,T) mark expansion of expression domain from VZ to pial surface in Neurog1/2^−/− OBs. Asterisks in (F,J,N,R) mark ectopic interneurons in Neurog2^KI/KI OBs. dTel, dorsal telencephalon; GL, glomerular layer; GCL, granule cell layer; MCL, mitral cell layer; OB, olfactory bulb; OBLS, olfactory bulb-like structure; ONL, olfactory nerve layer; vTel, ventral telencephalon; VZ, ventricular zone. Scale bars: 500 μm (A) to (T).