Figure 4

Defects in the differentiation of glutamatergic olfactory bulb neurons in Neurog1/2 double mutants. (A) to (H) Expression of Tbr1 (A) to (D) and Tbr2 (E) to (H) in the presumptive MCL of embryonic day (E) 13.5 wild-type (A,E), Neurog1^−/− (B,F), Neurog2 ^KI/KI (C,G) and Neurog1/2 ^−/− (D,H) OBs. White arrowheads in D,H mark the reduction in glutamatergic neurons in Neurog1/2^−/− OBs. White asterisks in (A) to (H) mark the ventricular zone. (I), (J) Quantitation of total numbers of Tbr1⁺ cells (I) and Tbr2⁺ cells (J) in E13.5 wild-type Neurog1^−/−, Neurog2^KI/KI and Neurog1/2^−/− OBs. Asterisks denote P <0.05. (K) to (N) Analysis of Neurog1 expression in E11.5 (K,L) and E13.5 (M,N) wild-type and Neurog2^−/− telencephalons. Arrows mark ectopic Neurog1 expression in (L) and (N). Scale bars: 250 μm (A) to (H), 1 mm (K) to (N).