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Figure 2 | Neural Development

Figure 2

From: Neurog1 and Neurog2 coordinately regulate development of the olfactory system

Figure 2

Defects in olfactory bulb morphogenesis and lamination in Neurog1−/−and Neurog1−/−;Neurog2KI/KImutant embryos. (A) to (D) Whole-mount dorsal views of partially dissected embryonic day (E) 18.5 wild-type (A), Neurog1−/− (B), Neurog2 KI/KI (C), and Neurog1/2 −/− (D) brains, all heterozygous or homozygous for a Neurog2GFPKI allele. Brains were left in the cranium and imaged by merging bright-field and GFP fluorescent images. Arrowheads mark the reduction in OB size in Neurog1−/− embryos (B), and apparent loss of the OB in Neurog1/2 −/− embryos (D). (E) to (H) GFP epifluorescence (green) and nuclear DAPI staining (blue) of sagittal sections through E18.5 wild-type (E), Neurog1−/− (F), Neurog2KI/KI (G), and Neurog1/2−/− (H) OBs. (I) to (L) H & E histological analysis of E18.5 wild-type (I,I'), Neurog1−/− (J,J'), Neurog2KI/KI (K,K'), and Neurog1/2−/− (L,L') OBs. (I') to (L') are twofold magnifications of the boxed areas in (I) to (L), respectively. GCL, granule cell layer; Gl, glomerular layer; MCL, mitral cell layer; NCX, neocortex; OB, olfactory bulb; OBLS, olfactory bulb-like structure; ONL, outer nerve layer; VZ, ventricular zone. Scale bars: 2 mm (A) to (D), 500 μm (E) to (L), 250 μm (I') to (L').

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