Figure 10

Normal OSN and OEC differentiation in Neurog1^−/−and Neurog2^−/−mutants, but apoptosis elevated in double mutants. (A) to (D) Expression of SCG10 in wild-type (A), Neurog1^−/− (B), Neurog2^−/− (C), and Neurog1/2^−/− (D) OBs at embryonic day (E) 18.5. (E) to (P) Co-labeling with DAPI (blue, E to P) and OMP (green, E to H), NCAM (green, I to L), GFP (green, M to P), p75 (red, I to L), or S100b (red, M to P) in wild-type (E,I,M), Neurog1^−/− (F,J,N), Neurog2^−/− (G,K,O), and Neurog1/2^−/− (H,L,P) OBs at E18.5. (Q) to (T) Co-labeling with DAPI (blue) and activated caspase 3 (AC3; red) in wild-type (Q), Neurog1^−/− (R), Neurog2^−/− (S), and Neurog1/2^−/− (T) OBs at E14.5. (U) to (W) Schematic representation of the three components of the olfactory system (U). The normal process of OSN innervation of the OB, and the subsequent formation of the glomerular layer by migrating OB interneurons (V) is perturbed in Neurog1/2^−/− embryos (W). OB, olfactory bulb; OE, olfactory epithelium; OEC, olfactory ensheathing cell; OSN, olfactory sensory neuron; PC, piriform cortex. Scale bars: 500 μm (A) to (P), 250 μm (Q) to (T).