Figure 5

Knocking down  cxcr5  with cerebroventricular microinjection (CVMI) results in reduced regenerative neurogenesis but not radial glial cell proliferation. (A) Lesion, CVMI and BrdU treatment scheme. (B) BrdU and S100β immunohistochemistry (IHC) with 4,6-diamidino-2-phenylindole (DAPI) counterstaining on control morpholino-injected brains. (B’) BrdU channel. (B”) S100β channel. (C) BrdU and S100β IHC with DAPI counterstaining on cxcr5 antisense morpholino-injected brains. (C’) BrdU channel. (C”) S100β channel. (D) Graph showing the average number of proliferating glial cells. Knocking down cxcr5 does not alter the levels of proliferating radial glial cells. (E) Lesion, CVMI, BrdU treatment and pulse-chase scheme. (F) BrdU and HuC IHC with DAPI counterstaining on control morpholino-injected brains. (F’) BrdU channel. (F”) HuC channel. (G) BrdU and HuC IHC with DAPI counterstaining on cxcr5 antisense morpholino-injected brains. (G’) BrdU channel. (G”) HuC channel. (H) Graph showing the average number of newborn neurons. Knocking down cxcr5 significantly reduces the levels of regenerative neurogenesis.