Lineage assessment after perinatal electroporation. (A) Cells electroporated with membrane EGFP and TagBFP are shown in the tectum, 14 days after electroporation. (B) Cells electroporated with membrane EGFP and TagBFP in the cerebral cortex are displayed, 14 days following electroporation. (C) Quantification of percentages of cells morphologically resembling neurons, astrocytes or miscellaneous populations is shown. (D) Electroporated cells with neuronal morphology express the interneuron-specific transcription factor, MafB. (E) Three-dimensional reconstruction and surface rendering of a GFP+ interneuron and (F) a GFP+ protoplasmic astrocyte using Imaris:Bitplane software. Scale bars measure 20 μm in A,B,D and 10 μm in E-F.