Early expression of glial markers following perinatal electroporation. (A-A3) Cells electroporated with membrane EGFP and TagBFP are Nestin+ after 2 days. Note the presence of Nestin within the membrane of the GFP+/TagBFP+ cell in the inset. (B-B4) EGFP+ (presumptive) glial cells did not stain for NG2. (C-C4) EGFP+ glial cells express Nfia and varying amounts of Gfap at 2 days post-EP. Scale bars measure 10 μm in (A3), (B4), and (C4).