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Figure 6 | Neural Development

Figure 6

From: Layer 6 cortical neurons require Reelin-Dab1 signaling for cellular orientation, Golgi deployment, and directed neurite growth into the marginal zone

Figure 6

Reelin-Dab1 signaling is required for Golgi extension. Sections from explants were immunolabeled with the cis- Golgi marker GM130 and imaged. (A) Scatter plots of proximal-to-distal Golgi lengths measured in each experimental condition. L6 neuron Golgi lengths were compact in both r/r Ct-CM and s/s Ct-CM explants as compared to elongated wt and partially rescued (i.e. elongated) Golgi in r/r Rln-CM explants. Rln-CM injection into s/s or wt explants(s/s Rln-CM and wt Rln-CM) had no effect on Golgi length in comparison to Ct-CM injections. (B) Bar graph representation of measured Golgi lengths. Dashed lines in scatter plots represent mean values; error bars denote standard error of the mean. Golgi lengths were compared across images acquired from 18 wt explants, 7 r/r Ct-CM explants, 5 s/s Ct-CM explants, 9 r/r Rln-CM explants, 5 s/s Rln-CM explants, and 6 wt Rln-CM explants. One-way ANOVA with post-hoc Bonferroni t-tests were performed between treatment conditions. *** p < 0.001, as compared to wt controls.

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Neural Development

ISSN: 1749-8104