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Figure 1 | Neural Development

Figure 1

From: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate

Figure 1

Presence of I h in cortical plate neurons at P0 and its developmental increase. (A) Left: A distinct sag was observed at P0 after current injection in ACSF. Right: Pharmacological isolation of Ih depolarized the membrane and the sag was still present. Application of ZD7288 abolished the sag (red trace). Membrane voltages were recorded in the presence of 100 μM intracellular cAMP. (B) and (C) Reduction of the inward current at P0 by Ih blockers. (B) Top: Pharmacological isolated Ih traces of a P0 neuron before (black) and 450 seconds after bath application of 50 μM ZD7288 (red). Bottom: ZD7288 sensitive current component obtained by subtracting the remaining current after ZD7288 application from the initial hyperpolarization induced current. (C) Top: Current after pharmacological isolation of Ih (black trace) and after bath application of 2 mM Cs+ for 360 seconds (blue trace). Bottom: Cs+ sensitive current obtained by the same method as described for (B). Currents were activated by stepping to a command voltage of −120 mV (B) or −130 mV (C) from a holding potential of −40 mV. Note that tail currents were also abolished by the respective blockers. Scale bars in (C) also apply to (B). Displayed currents were recorded in the intracellular presence of 100 μM cAMP. (D) Families of pharmacologically isolated and non-post hoc processed Ih (see Methods) in the absence of cAMP in neocortical neurons at developmental stages P0, P1 and P30 in response to 2-second voltage steps from −60 to −130 mV in 10 mV intervals, elicited from a holding potential of −40 mV (bottom). Note that traces depicted from P0 and P1 neurons are magnified approximately eightfold for clarity (see scale bars). Tail currents are cut at 500 ms, for a detailed analysis see Figures 2 and 4. The indicated time scale applies for all currents in (D).

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