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Figure 7 | Neural Development

Figure 7

From: Normal sulfation levels regulate spinal cord neural precursor cell proliferation and differentiation

Figure 7

Sodium chlorate attenuates neuronal polarization of neural precursor cell-derived neurons. (A-D, G) Photomicrographs of differentiated NPCs stained for cleaved Caspase3, MAP2, and Tau after seven days of differentiation. (A-D) NaClO3 treatment did not result in an increased apoptotic cell death after seven days. However, in line with an increased percentage of βIII-Tubulin-positive neurons after four days, the relative number of MAP2-positive cells was enhanced in response to NaClO3. (E) The quantification of MAP2-positive neurons revealed a significant increase in the relative amount of neurons in NaClO3 treated cultures (n = 4; P = 0.015). (F, G) Interestingly, the percentage of Tau-positive cells among MAP2-positive neurons was strongly reduced after NaClO3 treatment (n = 4; P = 0.002). To visualize the nuclei the cells were counterstained with Hoechst 33528. Scale bar: 50 μm (A-D), 25 μm (G).

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