Figure 2
From: Structural distinctions in BMPs underlie divergent signaling in spinal neurons
Generation of BMP conditioned medium (CM). (A-C) Western blots of rBMPs and 20-fold concentrated CM. (A) rBMP9 at the indicated concentrations and BMP9 CM probed with α-BMP9. (B) myc-tagged BMP CM probed with α-myc. (C) HA-tagged BMP CM probed with α-HA. In CM lanes, the lower bands represent BMP monomers migrating at 18 kDa for BMP7 and BMP6, 15 kDa for GDF7 and 13 kDa for BMP9. The upper bands represent glycosylated forms of the BMPs.