Notch misexpression leads to increased mitotic activity in culture. (A) Third instar larval brain of genotype insc-Gal4/+; tub-Gal80ts/UAS-Notchintra stained with anti-PH3 (red) and anti-Elav (blue). (B, C) Cell culture of genotype insc-Gal4/+; tub-Gal80
/UAS-Notchintra stained with anti-PH3 (red) and DAPI (blue). Cells maintained for one day (B) and four days (C) in culture.