Molecular markers of neural cells in third instar larval brains and primary cell cultures. Immunostainings are displayed together with the DNA-marker DAPI (red) to label the nuclei of all cells. (A) Shows one brain hemisphere, anti-Dpn (green) labels nuclei of NBs, Dlg labels cell cortices (red in A). (B) Shows Dpn-labeled progenitors in primary brain cultures. (C, D) Anti-Pros (green) stains the nuclei of both GMCs and neurons. (E, F) Anti-Elav (green) marks nuclei of postmitotic neurons. (G, H) In the brain anti-FasII labels pioneering axonal fascicles, in cultured neurons anti-FasII marks membranes of pioneering neurons. (I, J) Glial cells within brains and culture are marked by anti-Repo (green). All images are Z-projections of confocal stacks.