Figure 7
From: Wnt3 and Wnt3a are required for induction of the mid-diencephalic organizer in the caudal forebrain
Lack of Wnt signaling leads to apoptosis in the MDO. Lateral views and dorsal views of the left hemisphere (marked by ') of Shh::RFP transgenic embryos at 36 hpf. Wnt3/wnt3a morphant embryos as well as embryos treated with IWR1 display an increase of apoptotic cells within the MDO at 36 hpf (n = 4/6, A-C'). Upon blockage of Tp53 function apoptosis is down-regulated within the organizer tissue of wnt3/wnt3a morphant embryos as well as embryos treated with IWR1 and consistently, shh:RFP expression is restored (D-F').