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Figure 7 | Neural Development

Figure 7

From: Neurogenesis in the central olfactory pathway of adult decapod crustaceans: development of the neurogenic niche in the brains of procambarid crayfish

Figure 7

Neuroblast X, the cap and trail 9 (left side of the brain) in double-pulse experiments (48-h interval between the pulses) with the S-phase markers BrdU (first pulse, red) and EdU (second pulse, green) combined with immunolocalization of tyrosinated tubulin (white); late POI stages. (A) Neuroblast X (NB-X) and its progeny are embedded in tubulin immunoreactive material. A string of progeny extends towards trail 9. (B) Overview of the cap, NB-X, stream 10 and trail 9 close to the medial giant neuron (GN). Neuroblast Z is also shown as a landmark (Z). (C) The same specimen as in (A) but in a more superficial focus plane showing close association of the cap and NB-X, which both contribute progeny to trail 9. (D-F) Additional specimens showing NB-X and the associated trail of progeny towards cluster 9 (only the red channel of the first pulse is shown and tyrosinated tubulin in (F)). Scale bars: 10 μm (A), 20 μm (B, D), 50 μm (C), 10 μm (E, F).

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