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Figure 10 | Neural Development

Figure 10

From: Neurogenesis in the central olfactory pathway of adult decapod crustaceans: development of the neurogenic niche in the brains of procambarid crayfish

Figure 10

The deutocerebral proliferative system and protoniche (left side of the brain) in double-pulse experiments (7-day interval between the pulses) with the S-phase markers BrdU (first pulse, red) and EdU (second pulse, green) combined with immunolocalization of tyrosinated tubulin (white); animals were in POI stage for the first pulse and in the early POII stage for the second pulse. (A, B) Different focal planes of the same specimen. After 7 days, all cells labeled in the first pulse have moved out into clusters 10 and 9. The green cells in the upper right corner may be glia cells. The boxed area in (A) is magnified in (C). (C) Higher magnification of the protoniche. Note that the green fluorescence in the cytoplasm of the medial giant neuron (GN) most likely is background labeling. (D) Higher magnification of a deeper focal plane of the same specimen as in (A-C) reveals that the lateral proliferation zone (LPZ) is mitotically active in the second pulse (green). Unspecific background visualizes the accessory lobe (AL) and olfactory lobe (OL). Scale bars: 50 μm (A, B, D), 20 μm (C).

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